Mismatch between screen & work area location

I need your assistance, please. I want to start cutting larger layouts and when I tested the location of the head of my 6040 K40 CO2 laser running on Meerk40t I discovered that there is an offset difference both horizontally and vertically between the screen display work area and the location of the laser head.

How can I rectify this?

Could you be more specific please? Is this a constant offset, or one that is proportional with the position, ie for 2 inches it is less noticeable than at 20 inches.
For the first case you could define an offset value in the laser settings, for the second case you could define a correction factor.
So draw/burn a 10x10 inch rectangle and measure the length of the sides, the ratio between measured and expected length is your correction factor then.

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Sorry for the poor explanation, I meant that the physical location of the laser head is not the same as on the location on the screen. In other words, if I make a dot on x:y at 0:0 the actual position is at x:y 10:25 for example.

This difference looks like it is standard across the full size of the bed.

Hope this makes the explanation a bit more clear.

It is now a little more clear, but as with your previous questions, some pictures or videos would assist a lot as would some units-of-measure i.e. is 10:25 10microns,10microns 10/1000",25/1000", 10mm,25mm, 10miles,25miles?

But assuming that this is mm, and that you did a Home in MK before moving the head, then the explanation is that your physical origin is in the wrong place.

IMG_9137
IMG_9138
These two belong toghether at 0:0
IMG_9139
IMG_9140
These at at 600:400

Where’s your physical home, left top or bottom right, or…?

Physical home is Top right

The 0:0 location howerver is top left.

So, your scale is off then, afaics. Home your device, fire the laser, either via the physical laser panel or meerk40ts panel, move to meerk40ts 0,0 and fire again. Measure the physical distance between these two points and divide the virtual distance, ie your laser bed width by the measured distance, that’s your x scale factor.
If necessary do the equivalent for y.

On second thought: your bed dimensions are entered correctly?

I dont think this is the problem, as the design size of the item is exactly the same as the size of thr cut item.

How do I determine the correct size of the bed? I entered 600mm x 400mm - the size of the reported bed size on the advertising material.